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stat3 luciferase reporter assays  (BPS Bioscience)


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    BPS Bioscience stat3 luciferase reporter assays
    A. HCT116 (n= 10) or B. DLD-1 ± TSSK6-V5 (n=9) were transfected with siCTRL or siSTAT3 for 48 hours then plated into soft agar. 10-14 days later, samples were stained and quantitated. Bars represent mean ± s.d. C. 293Ts were transfected with <t>STAT3</t> reporter ± TSSK6-V5 (n=9). After 48 hours, vehicle control or 75 ng/uL Il-6 was added, and cells were incubated overnight. Dual Glo Luciferase assays were used to read STAT3 reporter firefly luminescence normalized to constitutively active renilla luminescence. Samples with IL-6 were analyzed with a paired t-test. D. Confocal images of DLD-1 ± TSSK6-V5 cells plated onto glass coverslips for 1 day prior to staining with STAT3. The scale bar is 20 µm. Nuclear STAT3 signal normalized to total STAT3 signal per cell was quantified using ImageJ. Data points represent individual cells from 3 fields of view across 4 coverslips. Paired t-test used for statistical analysis.
    Stat3 Luciferase Reporter Assays, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stat3+luciferase+reporter+assays/STAT3+Luciferase+Reporter+Lentivirus/bio_rxiv__64898__2026__03__10__710807-69-0-15
    Average 94 stars, based on 6 article reviews
    stat3 luciferase reporter assays - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Aberrant expression of the testis kinase TSSK6activates FAK–STAT3 signaling to promote tumorigenic growth"

    Article Title: Aberrant expression of the testis kinase TSSK6activates FAK–STAT3 signaling to promote tumorigenic growth

    Journal: bioRxiv

    doi: 10.64898/2026.03.10.710807

    A. HCT116 (n= 10) or B. DLD-1 ± TSSK6-V5 (n=9) were transfected with siCTRL or siSTAT3 for 48 hours then plated into soft agar. 10-14 days later, samples were stained and quantitated. Bars represent mean ± s.d. C. 293Ts were transfected with STAT3 reporter ± TSSK6-V5 (n=9). After 48 hours, vehicle control or 75 ng/uL Il-6 was added, and cells were incubated overnight. Dual Glo Luciferase assays were used to read STAT3 reporter firefly luminescence normalized to constitutively active renilla luminescence. Samples with IL-6 were analyzed with a paired t-test. D. Confocal images of DLD-1 ± TSSK6-V5 cells plated onto glass coverslips for 1 day prior to staining with STAT3. The scale bar is 20 µm. Nuclear STAT3 signal normalized to total STAT3 signal per cell was quantified using ImageJ. Data points represent individual cells from 3 fields of view across 4 coverslips. Paired t-test used for statistical analysis.
    Figure Legend Snippet: A. HCT116 (n= 10) or B. DLD-1 ± TSSK6-V5 (n=9) were transfected with siCTRL or siSTAT3 for 48 hours then plated into soft agar. 10-14 days later, samples were stained and quantitated. Bars represent mean ± s.d. C. 293Ts were transfected with STAT3 reporter ± TSSK6-V5 (n=9). After 48 hours, vehicle control or 75 ng/uL Il-6 was added, and cells were incubated overnight. Dual Glo Luciferase assays were used to read STAT3 reporter firefly luminescence normalized to constitutively active renilla luminescence. Samples with IL-6 were analyzed with a paired t-test. D. Confocal images of DLD-1 ± TSSK6-V5 cells plated onto glass coverslips for 1 day prior to staining with STAT3. The scale bar is 20 µm. Nuclear STAT3 signal normalized to total STAT3 signal per cell was quantified using ImageJ. Data points represent individual cells from 3 fields of view across 4 coverslips. Paired t-test used for statistical analysis.

    Techniques Used: Transfection, Staining, Control, Incubation, Luciferase

    A. Volcano plots from differential gene expression analysis from loss-of-function (HCT116) and gain-of-function (HCEC) RNA-sequencing. Vertical dotted lines indicate log2(fold change) equals -0.5 or 0.5 and horizontal dotted lines indicate –log10(p-value) equals 2. Data points below this horizontal dotted line are not shown. B. Datasets returned in the Top Ten of Enrichr’s Pathways suite of databases as being enriched for in gain-of-function (HCEC, log2(fold change)>0.5 and p-value<0.01) and loss-of-function (HCT116, log2(fold change) <-0.5 and p-value<0.01) differential gene expression analysis. C. STAT3 datasests returned from Enrichr Transcription suite of databases as being enriched for in gain-of-function (HCEC, log2(fold change)>0.5 and p-value<0.01) and loss-of-function (HCT116, log2(fold change) <-0.5 and p-value<0.01) differential gene expression analysis. D. HCT116 were transfected with indicated siRNA for 48 hours then plated into soft agar. 10-14 days later, samples were stained and quantitated.
    Figure Legend Snippet: A. Volcano plots from differential gene expression analysis from loss-of-function (HCT116) and gain-of-function (HCEC) RNA-sequencing. Vertical dotted lines indicate log2(fold change) equals -0.5 or 0.5 and horizontal dotted lines indicate –log10(p-value) equals 2. Data points below this horizontal dotted line are not shown. B. Datasets returned in the Top Ten of Enrichr’s Pathways suite of databases as being enriched for in gain-of-function (HCEC, log2(fold change)>0.5 and p-value<0.01) and loss-of-function (HCT116, log2(fold change) <-0.5 and p-value<0.01) differential gene expression analysis. C. STAT3 datasests returned from Enrichr Transcription suite of databases as being enriched for in gain-of-function (HCEC, log2(fold change)>0.5 and p-value<0.01) and loss-of-function (HCT116, log2(fold change) <-0.5 and p-value<0.01) differential gene expression analysis. D. HCT116 were transfected with indicated siRNA for 48 hours then plated into soft agar. 10-14 days later, samples were stained and quantitated.

    Techniques Used: Gene Expression, RNA Sequencing, Transfection, Staining

    Related Articles

    Luciferase:

    Article Title: Aberrant expression of the testis kinase TSSK6activates FAK–STAT3 signaling to promote tumorigenic growth
    Article Snippet: Images were captured with a Leica S9D dissecting microscope and quantitated by ImageJ software. .. STAT3 Luciferase Reporter Assays were performed in 293T cells using a STAT3 Reporter Kit (Cat#79730, BPS Bioscience) as described by the manufacturer. ..



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    Image Search Results


    A. HCT116 (n= 10) or B. DLD-1 ± TSSK6-V5 (n=9) were transfected with siCTRL or siSTAT3 for 48 hours then plated into soft agar. 10-14 days later, samples were stained and quantitated. Bars represent mean ± s.d. C. 293Ts were transfected with STAT3 reporter ± TSSK6-V5 (n=9). After 48 hours, vehicle control or 75 ng/uL Il-6 was added, and cells were incubated overnight. Dual Glo Luciferase assays were used to read STAT3 reporter firefly luminescence normalized to constitutively active renilla luminescence. Samples with IL-6 were analyzed with a paired t-test. D. Confocal images of DLD-1 ± TSSK6-V5 cells plated onto glass coverslips for 1 day prior to staining with STAT3. The scale bar is 20 µm. Nuclear STAT3 signal normalized to total STAT3 signal per cell was quantified using ImageJ. Data points represent individual cells from 3 fields of view across 4 coverslips. Paired t-test used for statistical analysis.

    Journal: bioRxiv

    Article Title: Aberrant expression of the testis kinase TSSK6activates FAK–STAT3 signaling to promote tumorigenic growth

    doi: 10.64898/2026.03.10.710807

    Figure Lengend Snippet: A. HCT116 (n= 10) or B. DLD-1 ± TSSK6-V5 (n=9) were transfected with siCTRL or siSTAT3 for 48 hours then plated into soft agar. 10-14 days later, samples were stained and quantitated. Bars represent mean ± s.d. C. 293Ts were transfected with STAT3 reporter ± TSSK6-V5 (n=9). After 48 hours, vehicle control or 75 ng/uL Il-6 was added, and cells were incubated overnight. Dual Glo Luciferase assays were used to read STAT3 reporter firefly luminescence normalized to constitutively active renilla luminescence. Samples with IL-6 were analyzed with a paired t-test. D. Confocal images of DLD-1 ± TSSK6-V5 cells plated onto glass coverslips for 1 day prior to staining with STAT3. The scale bar is 20 µm. Nuclear STAT3 signal normalized to total STAT3 signal per cell was quantified using ImageJ. Data points represent individual cells from 3 fields of view across 4 coverslips. Paired t-test used for statistical analysis.

    Article Snippet: STAT3 Luciferase Reporter Assays were performed in 293T cells using a STAT3 Reporter Kit (Cat#79730, BPS Bioscience) as described by the manufacturer.

    Techniques: Transfection, Staining, Control, Incubation, Luciferase

    A. Volcano plots from differential gene expression analysis from loss-of-function (HCT116) and gain-of-function (HCEC) RNA-sequencing. Vertical dotted lines indicate log2(fold change) equals -0.5 or 0.5 and horizontal dotted lines indicate –log10(p-value) equals 2. Data points below this horizontal dotted line are not shown. B. Datasets returned in the Top Ten of Enrichr’s Pathways suite of databases as being enriched for in gain-of-function (HCEC, log2(fold change)>0.5 and p-value<0.01) and loss-of-function (HCT116, log2(fold change) <-0.5 and p-value<0.01) differential gene expression analysis. C. STAT3 datasests returned from Enrichr Transcription suite of databases as being enriched for in gain-of-function (HCEC, log2(fold change)>0.5 and p-value<0.01) and loss-of-function (HCT116, log2(fold change) <-0.5 and p-value<0.01) differential gene expression analysis. D. HCT116 were transfected with indicated siRNA for 48 hours then plated into soft agar. 10-14 days later, samples were stained and quantitated.

    Journal: bioRxiv

    Article Title: Aberrant expression of the testis kinase TSSK6activates FAK–STAT3 signaling to promote tumorigenic growth

    doi: 10.64898/2026.03.10.710807

    Figure Lengend Snippet: A. Volcano plots from differential gene expression analysis from loss-of-function (HCT116) and gain-of-function (HCEC) RNA-sequencing. Vertical dotted lines indicate log2(fold change) equals -0.5 or 0.5 and horizontal dotted lines indicate –log10(p-value) equals 2. Data points below this horizontal dotted line are not shown. B. Datasets returned in the Top Ten of Enrichr’s Pathways suite of databases as being enriched for in gain-of-function (HCEC, log2(fold change)>0.5 and p-value<0.01) and loss-of-function (HCT116, log2(fold change) <-0.5 and p-value<0.01) differential gene expression analysis. C. STAT3 datasests returned from Enrichr Transcription suite of databases as being enriched for in gain-of-function (HCEC, log2(fold change)>0.5 and p-value<0.01) and loss-of-function (HCT116, log2(fold change) <-0.5 and p-value<0.01) differential gene expression analysis. D. HCT116 were transfected with indicated siRNA for 48 hours then plated into soft agar. 10-14 days later, samples were stained and quantitated.

    Article Snippet: STAT3 Luciferase Reporter Assays were performed in 293T cells using a STAT3 Reporter Kit (Cat#79730, BPS Bioscience) as described by the manufacturer.

    Techniques: Gene Expression, RNA Sequencing, Transfection, Staining